Application of accelerated solvent extraction in evaluation of Rosa Canina L. antioxidant capacity

Polyphenolic compounds are the main component in vegetal samples. These natural compounds have bio-active properties making them valuable across multiple industries. One of the primary benefits of polyphenols compounds is their strong anti-oxidant activity, acting as preventing or scavenging free radicals induced oxidative damaging. Therefore, the development of extraction methods that preserve the bio-activity of polyphenolic compounds while ensuring safety and sustainability is essential for achieving a balance between industrial development and ecological responsibility. The aim of our work was to study the efficiency of the Accelerated Solvent Extraction (ASE), a green technology, on Rosa canina L. fruits, rich in bio-active compounds. The polyphenolic compounds profile of extracts had different composition depending on solvent composition, extraction time and sample amount, but the order of the analysed polyphenolic compounds had been preserved, namely ellagic acid > quercitrin > quercetin 3-D-glucoside > catechin > chlorogenic acid > gallic acid > rutin > quercetin > kaempferol. Also, antioxidant activity was strongly affected by ASE operational parameters. When an aqueous medium was used, the antioxidant activity had higher values by 35%. Growing the extraction time by 3 times had led to an increase in the antioxidant activity by 1.5 times. Leveraging ASE optimizes resource use, achieving comparable yields of essential nutrients, vitamins, and antioxidants from rosehip with significantly reduced solvent and energy consumption. This method enhances extraction efficiency and aligns with sustainability goals by minimizing waste and environmental impact. As demand for natural products grows, ASE emerges as a promising approach to maximize the quality and yield of extracts while conserving vital resources.